Plasma Membrane Fraction Western Blot Cocktail
ab139413 contains 3 mouse monoclonal antibodies each targeting a specific organelle marker. The presence of plasma membrane is determined by Anti-Sodium Potassium ATPase antibody; cytosol by Anti-GAPDH; and nucleus by Anti-Histone H3 (di methyl K9). This cocktail is suitable for determining the purity of organelle isolates prior to further characterization. This product is particularly valuable to researchers working in organelle proteomics.
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Specification for Plasma Membrane Fraction Western Blot Cocktail
Specifications Product :
Sample type :Tissue Extracts, Cell Lysate, Tissue Homogenate, Nuclear Extracts
Assay type :Indirect
Species reactivity :Reacts with: Mouse, Rat, Human
Storage instructions :Store at +4°C. Please refer to protocols
Cellular localization :Sodium Potassium ATPase: Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. GAPDH: Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear
| Component | 200µl |
| 250X Plasma Membrane Fraction WB Cocktail | 1 x 200µl |