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NucleoSpin RNA, Mini kit for RNA purification
NucleoSpin®RNA kits are recommended for the isolation of RNA from cultured cells and tissue. Support protocols for the isolation of RNA from reaction mixtures, bacteria, and yeasts using the NucleoSpin®RNA kit are included. The kits allow purification of pure RNA with an A260/A280 ratio generally exceeding 1.9 (measured in TE buffer, pH7.5).
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Specification for NucleoSpin RNA, Mini kit for RNA purification
Mini spin kit for isolation of RNA of highest integrity
| Application | Isolation of RNA |
| Target | RNA |
| CE certified | No, research use only |
| Technology | Silica membrane technology |
| Brand | NucleoSpin |
| Format | Mini prep |
| Package unit | 10 Prep(s), 50 Prep(s), 250 Prep(s) |
| Handling | Centrifugation, vacuum |
| Lysate clarification | Centrifugation, NucleoSpin Filter |
| Automated use | No |
| Sample material | Animal tissue, Bacteria, Cells, Human tissue, Yeast |
| Sample amount | < 5 x 10⁶ cultured cells, < 30 mg human / animal tissue, < 10⁹ bacterial cells, < 10⁸ yeast cells |
| Fragment size | > 200 nt |
| Typical yield | 14 µg (10⁶ HeLa cells), 70 µg (10⁹ bacterial cells) |
| Theoretical binding capacity | 200 µg |
| Typical purity A260/A280 | 1.9–2.1 |
| Elution volume | 30–120 µL |
| Preparation time | 35 min/6 preps |
| Typical downstream application | RNA-Seq, RT−PCR |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 24 Months |
| Hazardous material | Yes |