StemMACS™ MSC Expansion Media Kit XF, human
The StemMACS MSC Expansion Media Kit is an optimized and standardized serum- and xeno-free medium for the reproducible and reliable expansion and enumeration of MSCs from human BM samples or other tissue sources, while maintaining the cells' multi-lineage differentiation potential. StemMACS MSC Expansion Media Kit XF is completely free of non-human animal derived components and does not require the use of cell attachment substrates.
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Product data and images for StemMACS™ MSC Expansion Media Kit XF, human
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MSCs cultured in StemMACS MSC Expansion Media XF show an increased clonogenic potential (A) as well as cell proliferation rate (B) compared to MSCs cultured in ?MEM + 5% platelet lysate or when cultured in 10% fetal calf serum (FCS)–containing media.
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Human MSCs cultured in StemMACS MSC Expansion Media XF were differentiated towards osteogenic (A), adipogenic (B), or chondrogenic (C) lineage using StemMACS MSC Diff Media. Cells cultured in StemMACS MSC Expansion Media XF maintain the broad differentiation potential.
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MSCs cultured in StemMACS MSC Expansion Media XF exhibit immunomodulating properties. The immunomodulation was measured using the MSC Suppression Inspector by cell proliferation of responder T (Tresp) cells in co-culture with MSCs.
Specification for StemMACS™ MSC Expansion Media Kit XF, human
The formulation of StemMACS MSC Expansion Media Kit XF was designed to efficiently support the expansion of MSCs in vitro while maintaining their differentiation potential. The media formulation is xeno- and serum-free and is manufactured under strictly controlled conditions using ingredients of the highest quality. StemMACS Expansion Media Kit XF offers consistent lot-to-lot performance and optimal conditions for the cultivation of MSCs. It is suitable for the derivation, expansion, and enumeration using the colony-forming–unit fibroblast (CFU-F) assay and does not require the use of any cell attachment substrate for optimal performance.