MSC-Brew GMP Medium
MSC-Brew GMP Medium is an optimized and standardized medium for the generation and expansion of mesenchymal stem cells (MSCs) from human bone marrow (BM) or other tissue sources. The formulation of MSC-Brew GMP Medium is designed to efficiently support the expansion of MSCs in vitro while maintaining their differentiation potential. The medium is xeno- and serum-free and is manufactured under strictly controlled conditions using ingredients of the highest quality. MSC-Brew GMP Medium offers consistent lot-to-lot performance and optimal conditions for the cultivation of MSCs.
ReadmoreProduct data and images for MSC-Brew GMP Medium
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Comparison of MSC-Brew GMP Medium to other media compositions used for cell manufacturing reveals advantageous growth kinetics when MSC-Brew GMP Medium is used. Clinically relevant cell numbers are reached within a shorter time frame (14 days faster), which in turn results in less media consumption. Overall media consumption can be reduced up to 70%.
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MSC-Brew GMP Medium supports expansion of MSCs derived from human bone marrow, human adipose tissue, and human umbilical cord. Human bone marrow–derived (BM-MSC), adipose tissue–derived (AT-MSC), and umbilical cord–derived MSCs (UC-MSC) were cultured in MSC-Brew GMP Medium.
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The differentiation potential of MSCs was assessed for adipocytes (A), chondrocytes (B), and osteoblasts (C). MSCs were cultivated in StemMACS AdipoDiff Medium for18 days and adipocyte differentiation was analyzed using a lipid-staining dye (red) to demonstrate lipid accumulation. Nuclei were detected with Dapi (blue). After 26 days of culture in StemMACS ChondroDiff Medium, chondrocyte differentiation was analyzed using an anti-aggrecan antibody (red) for detection of proteoglycan. Nuclei were detected with Dapi (blue). After 10 days of culture in StemMACS OsteoDiff Medium, osteoblast differentiation was analyzed by detection of alkaline phosphatase activity.
Specification for MSC-Brew GMP Medium
Background information
MSC-Brew GMP Medium is based on the formulation of StemMACS MSC Expansion Media Kit XF thus enabling seamless translation from research to clinical applications.
- Serum-free and xeno-free formulation
- Manufactured under strictly controlled conditions
- Consistent lot-to-lot performance
- Quality control: functionality test on every batch
- 500 mL or 2000 mL bags – without phenol red
Applications
The MSC-Brew GMP Medium for the reproducible and reliable isolation and expansion of MSCs from human bone marrow and other human tissues, such as adipose tissue and umbilical cord.
Disclaimer
MACS GMP Products are for research use and ex vivo cell culture processing only, and are not intended for human in vivo applications.