Read modes

• Absorbance
• Fluorescence (top/bottom read)
• Luminescence (top read)
• Time-Resolved Fluorescence (top read)
• Fluorescence Polarization (top read)
• HTRF (top read)
• AlphaScreen (top read)

Wavelength ranges

• Abs: 230 - 1000 nm
• FL Ex: 250 - 830 nm
• FL Em: 270 - 850 nm
• Lumi: 300 - 850 nm

Wavelength selection

• Cartridge filters and monochromator tunable in 1 nm increments

Absorbance photometric accuracy/linearity

• < ±0.010 OD ±1.0%, 0 to 2 OD

Absorbance photometric precision/repeatability

• < ±0.003 OD ±1.0%, 0 to 2 OD

Sensitivity top read optimized

• FL: Mono: 0.5 pM fluorescein 96 well, 1 pM fluorescein 384 well; Cartridge: 0.1 fmol/well in 75 μL fluorescein 384 well, 0.03 fmol/well in 8 μL fluorescein 1536 well
• Lumi: Mono: 3 pM ATP-Glow 96 well, 6 pM ATP-Glow 384 well; Cartridge: 20 amol ATP-flash
• TRF cartridge: 3 amol/well in 100 μL europium (0.03 pM) 384 well, 1 amol/well in 8 μL europium (0.125 pM) 1535 well
• FP cartridge: 3 mP (75 μl) fluorescein 384 well, 6 mP (8μl) fluorescein 1536 well
• AlphaScreen cartridge: <100 amol/well 384 well

Light source(s)

• High power xenon flash lamp
• LEDs in visible range

Detector(s)

• Silicon photodiode
• Cooled photomultiplier tube

Read times

• Abs: 29 seconds
• FL: 22 seconds
• Lumi: 21 seconds
• TRF: 21 seconds
• FP: 38 seconds
• AS: 22 seconds

Plate shaking

• Linear
• Orbital

Plate type(s)

• 6-1536 well plates

Temperature control

• Ambient + 4°C to 45°C

Dimensions

• H 32.5 cm x W 39.2 cm x D 60.55 cm

Validation tools

• Absorbance validation plate
• Fluorescence validation plate
• Luminescence validation plate
• Multi-mode validation plate
• SoftMax® Pro GxP Software