qPCRBIO cDNA Synthesis Kit (25 x 20μL Reactions)
High quality cDNA synthesis is essential for downstream real-time PCR analysis and successful expression studies. qPCRBIO cDNA Synthesis Kit is an easy-to-use 2 tube system specifically developed to generate cDNA for use in real-time PCR. The reverse transcriptase, buffer system and optimised blend of random hexamers with anchored oligo(dT) primers provide unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations.
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qPCRBIO cDNA Synthesis Kit was used for cDNA synthesis using a 10 fold serial dilution of mouse total RNA from 40pg to 400ng. qPCR was performed using qPCRBIO SyGreen Mix amplifying a 122bp fragment of the mouse ACTG gene. Efficiency was measured at 96% across the range tested.
Specification for qPCRBIO cDNA Synthesis Kit (25 x 20μL Reactions)
Specifications Product
| Component | 25 Reactions | 100 Reactions |
| 5x cDNA Synthesis Mix | 1 x 100µL | 1 x 400µL |
| 20x RTase (with RNase inhibitor) | 1 x 25µL | 1 x 100µL |
Storage
On arrival the kit should be stored between -30°C and -15°C. If stored correctly the kit will retain full activity for 12 months.