PCRBIO VeriFi™ Polymerase (100 Units)
PCRBIO VeriFi™ Polymerase is a versatile and robust high fidelity enzyme engineered for all PCR applications where greater sequence accuracy is required. Improved DNA binding and increased processivity result in shorter extension times, higher yields and the ability to amplify longer and more difficult targets.
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PCRBIO VeriFi Polymerase amplifies the range of fragment lengths indicated with high yield and specificity. The starting template amount is 4-30ng of mouse or human genomic DNA, diluted 1.5 to 3 fold. GC content ranges from 37-55%.
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Amplification of a 17.5kb fragment of the HBB gene. The starting template amount is 150ng (A and C) and 30ng (B and D) of human genomic DNA, diluted 2 fold. A 2-step PCR protocol was used with amplification at 72°C (A and B) or 68°C (C and D). GC content is 37%. PCRBIO VeriFi Polymerase amplifies long fragments with yields comparable to Takara PrimeSTAR GXL DNA Polymerase. L: PCRBIO Ladder II.
Specification for PCRBIO VeriFi™ Polymerase (100 Units)
PCRBIO VeriFi™Polymerase |
Component : 100 Units PCRBIO VeriFi™ Polymerase (2u/μL) 1 x 50μL 5x PCRBIO VeriFi™ Buffer 1 x 1.7mL 10x VeriMax Enhancer 1 x 1.7mL |