NGSBIO Library Quant Kit for Illumina® Separate-ROX (500 x 20μL Reactions)
The NGSBIO Library Quant Kit offers a reliable qPCR-based method for the quantification of libraries prepared for Illumina® NGS systems. The kit includes 5 DNA standards, primers specific to the P5 and P7 Illumina® adapter sequences and qPCRBIO SyGreen Mix. The advanced qPCR buffer system has been developed to ensure efficient amplification of all your libraries, including those that are GC or AT-rich. The kit is also supplied with a convenient library dilution buffer.
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Specification for NGSBIO Library Quant Kit for Illumina® Separate-ROX (500 x 20μL Reactions)
NGSBIO Library Quant Kit for Illumina® Lo-ROX
Component 100 Reactions 500 Reactions
2x qPCRBIO SyGreen Mix Lo-ROX 1 x 1mL 5 x 1mL
DNA Standards 1-5 30μL each 85μL each
10x Illumina® Primers 1 x 0.2mL 1 x 1mL
10x Dilution Buffer 1 x 0.6mL 2 x 1.5mL
NGSBIO Library Quant Kit for Illumina® Hi-ROX
Component 100 Reactions 500 Reactions
2x qPCRBIO SyGreen Mix Hi-ROX 1 x 1mL 5 x 1mL
DNA Standards 1-5 30μL each 85μL each
10x Illumina® Primers 1 x 0.2mL 1 x 1mL
10x Dilution Buffer 1 x 0.6mL 2 x 1.5mL
NGSBIO Library Quant Kit for Illumina® Separate-ROX
Component 100 Reactions 500 Reactions
2x qPCRBIO SyGreen Mix No-ROX 1 x 1mL 5 x 1mL
50μM ROX Additive 1 x 200μL 1 x 200μL
DNA Standards 1-5 30μL each 85μL each
10x Illumina® Primers 1 x 0.2mL 1 x 1mL
10x Dilution Buffer 1 x 0.6mL 2 x 1.5mL
NGSBIO DNA Standards for Illumina®
Component 500 Reactions
DNA Standards 1-5 85μL each
Reaction Volume
20μL
Storage
On arrival, products should be stored between -30 and -15°C. If stored correctly the kit will retain full activity for 12 months.